Puzzle master Professor Layton and his apprentice, Luke, have stumbled upon another mystery. It's up to them to navigate their way through numerous puzzles to determine who – or what – caused the death of Professor Layton's mentor, Dr. Schrader. Was it the mysterious Elysian Box, rumored to kill all who open it? Professor Layton and Luke find a clue that begins their adventure: a train ticket for the Molentary Express.

• Professor Layton and the Diabolical Box features a riveting new mystery and more than 150 new brain teasers, riddles and logic puzzles to challenge and delight players.
• Beyond the puzzles found in the game, players with a broadband Internet connection who connect their Nintendo DS and Nintendo DSi systems to Nintendo Wi-Fi Connection will get access to downloadable bonus puzzles. A new puzzle will be available each week for 33 weeks after the game launches.
• The new game includes a significantly greater amount of voice work and animated movie scenes compared to the original.